Composite

Part:BBa_K2064003:Design

Designed by: Dragos S. Chiriac   Group: iGEM16_Queens_Canada   (2016-10-21)


TyrC Acceptor COM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 32
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 237
    Illegal BsaI.rc site found at 216


Design Notes

Xba1 cut site removed, originally located downstream of the promoter


Source

Synthetic, codon optimized for E. coli

References